LOCUS       sequence-117949-        1124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-regulated mammalian expression vector for expressing 
            shRNA against human SADB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Alvarado-Kristensson M, Rodriguez MJ, Silio V, Valpuesta JM, Carrera
            AC
  TITLE     SADB phosphorylation of gamma-tubulin regulates centrosome 
            duplication.
  JOURNAL   Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 
            2009 Aug 2.
  PUBMED    19648910
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1921"; 
            journalName: "Nat Cell Biol"; date: "2009-09"; volume: "11"; issue: 
            "9"; pages: "1081-92"
COMMENT     Sequence Label: pTER SADB shRNA Human
FEATURES             Location/Qualifiers
     source          1..1124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    138..156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(202..221)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        423..527
                     /gene="bla"
                     /label=AmpR promoter
     CDS             712..1090
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(746..765)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agcggccgcc actgtggctg gatatctgca gaattccacc acactggact agtggatccg
       61 agctcggtac caagcttttc caaaaagctc cacgacgtct acgagtctct tgaactcgta
      121 gacgtcgtgg agcgggatct ctatcactga tagggaactt ataagattcc caaatccaaa
      181 gacatttcac gtttatggtg atttcccaga acacatagcg acatgcaaat attgcagggc
      241 gccactcccc tgtccctcac agccatcttc ctgccagggc gcacgcgcgc tgggtgttcc
      301 cgcctagtga cactgggccc gcgattcctt ggagcgggtt gatgacgtca gcgttcgaat
      361 tcctgcagcc cgggggatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg
      421 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      481 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      541 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      601 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      661 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      721 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      781 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      841 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      901 taaccatgag tgataacact gcggccaact ttacttctga caacgatcgg aggaccgaag
      961 gagctaaccg ctttttgcac aacatggggg gatcatgtaa ctcgccttga tcgttgggaa
     1021 ccggagctga atgaagccat accaacgacg agcgtgaccc cacgatgcct gtagcaatgg
     1081 caacaacgtt gcccaaacta ttaactgggg aactacttac tcta
//
