LOCUS       sequence-117951-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA targeting human ARF5a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Volpicelli-Daley LA, Li Y, Zhang CJ, Kahn RA
  TITLE     Isoform-selective effects of the depletion of ADP-ribosylation 
            factors 1-5 on membrane traffic.
  JOURNAL   Mol Biol Cell. 2005 Oct;16(10):4495-508. Epub 2005 Jul 19.
  PUBMED    16030262
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2005-10"; volume: "16(10)"; pages: "4495-508. Epub 
            2005 Jul 19"
COMMENT     Sequence Label: pSUPER-ARF5a
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(110..126)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(111..127)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(155..173)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(194..210)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(194..210)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(207..229)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    218..234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(242..248,249..266,267..272))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    287..308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(425..442)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(676..695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggatttgg gatcttataa gttctgtatg agaccacaga tcccctctgc tgatgaactc
       61 cagattcaag agatctggag ttcatcagca gatttttgga aaagcttatc gataccgtcg
      121 acctcgaggg ggggcccggt accagctttt gttcccttta gtgagggtta atttcgagct
      181 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      241 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      301 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      361 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      421 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      481 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      541 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      601 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      661 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      721 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      781 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      841 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      901 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      961 gga
//
