LOCUS       sequence-117954-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSG5 containing N-terminally RFP tagged mouse SADBs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Alvarado-Kristensson M, Rodriguez MJ, Silio V, Valpuesta JM, Carrera
            AC
  TITLE     SADB phosphorylation of gamma-tubulin regulates centrosome 
            duplication.
  JOURNAL   Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 
            2009 Aug 2.
  PUBMED    19648910
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1921"; 
            journalName: "Nat Cell Biol"; date: "2009-09"; volume: "11"; issue: 
            "9"; pages: "1081-92"
COMMENT     Sequence Label: pSG5 N-RFP SADBs
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..104
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     132..151
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        132..150
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             167..838
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TG"
     CDS             167..838
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TG"
     primer_bind     complement(297..315)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     565..584
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     759..782
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct
       61 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
      121 gcaaagaatt gtaatacgac tcactatagg gcgaattgat atcaccatgg cctcctccga
      181 ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca
      241 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      301 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      361 ccagtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      421 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      481 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
      541 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
      601 ctccaccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agatgaggct
      661 gaagctgaag gacggcggcc actacgacgc cgaggtcaag accacctaca tggccaagaa
      721 gcccgtgcag ctgcccggcg cctacaagac cgacatcaag ctggacatca cctcccacaa
      781 cgaggactac accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgg
      841 cccgaattcg gaccaggcac catgtcgtcc gggtccaagg aaggtggcgg gggctccc
//
