LOCUS       sequence-117958-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express the tetracycline
            transactivator tTA2 from the CAG promoter in a Cre-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV pCAG-FLEX2-tTA2-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    43..76
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    139..172
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(198..308)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 ccgcgggccc cgcgtggacg gtaccggcgc gccaccatgg ccataacttc gtataaagta
       61 tcctatacga agttatatca aaataggaag accaatgctt caccatcgac ccgaattgcc
      121 aagcatcacc atcgacccat aacttcgtat aatgtatgct atacgaagtt atgtttaaac
      181 ttaattaatc atccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc
      241 atatcaaggt caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca
      301 agggcgtcgg ccggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt
      361 agttccaggc cgaaaaggaa ggcaggttcg gctccctgat ggtcgaacag ctcaattgct
      421 tgtctcagaa gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact
      481 tgatgctcct gatcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg
      541 tacagtgcgt tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg
      601 gtttcgtact gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt
      661 gagaggagag cacagcggaa tgacttggcg ttgttccgca gaaagtcttg ccatgactcg
      721 ccttccagg
//
