LOCUS       sequence-117959-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for human KCC2 under CAG promoter. 
            Coexpression of TdTomato with IRES sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Bortone D, Polleux F
  TITLE     KCC2 expression promotes the termination of cortical interneuron 
            migration in a voltage-sensitive calcium-dependent manner.
  JOURNAL   Neuron. 2009 Apr 16;62(1):53-71. doi: 10.1016/j.neuron.2009.01.034.
  PUBMED    19376067
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.01"; journalName: "Neuron"; date: 
            "2009-04-16- 16"; volume: "62"; issue: "1"; pages: "53-71"
COMMENT     Sequence Label: pCITF-KCC2
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             517..543
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(719..736)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ctgaacgtcc cagaagagac ggctggtgac agtgaagaga agccagagga ggaggtgcag
       61 ctgatccacg atcagagtgc tcccagctgc cccagcagct ccccgtcccc aggggaggag
      121 cctgaggggg aaggggagac agatccggag aaggtgcatc tcacctggac caaggacaag
      181 tcggtggcag agaagaataa gggccccagt cctgtctcct ctgagggcat caaggacttc
      241 ttcagcatga agccggagtg ggagaacttg aaccagtcca acgtgcggcg catgcacacg
      301 gccgtgcggc tgaacgaggt catcgtgaag aaatcccggg acgccaagct tgttttgctc
      361 aacatgcctg ggcctccccg caaccgcaat ggtgatgaaa actacatgga gtttctcgag
      421 gtcctcacag agcacctgga ccgggtgatg ctggtccgcg gcggcggccg cgaggtcatc
      481 accatctact cctgagaacc aggtctaggg gatccatacc catacgatgt tccagattac
      541 gcatagatcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
      601 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
      661 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
      721 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
      781 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
      841 gacaggtgcc tctgcggcca aaagccacgt gt
//
