LOCUS       sequence-117961-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express the tetracycline
            transactivator tTA2 from the CAG promoter in a Cre-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV pCAG-FLEX2-tTA2-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    64..97
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    168..201
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttagatct gatatcccgc caccatggat
       61 atcataactt cgtataatgt atgctatacg aagttatggg tcgatggtga aatctggact
      121 agagggtcga tggtgatgct tggatcaaaa taggaagaat cccaaagata acttcgtata
      181 aagtatccta tacgaagtta tcgactagtg ctagactgga caagagcaaa gtcataaact
      241 ctgctctgga attactcaat gaagtcggta tcgaaggcct gacgacaagg aaactcgctc
      301 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
      361 tcgatgccct ggcaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
      421 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc attccgctgt gctctcctct
      481 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
      541 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
      601 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggat caggagcatc
      661 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccc
//
