LOCUS       sequence-117962-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminally HA tagged mouse SADBs containing substitutions 
            providing resistance to an shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Alvarado-Kristensson M, Rodriguez MJ, Silio V, Valpuesta JM, Carrera
            AC
  TITLE     SADB phosphorylation of gamma-tubulin regulates centrosome 
            duplication.
  JOURNAL   Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 
            2009 Aug 2.
  PUBMED    19648910
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1921"; 
            journalName: "Nat Cell Biol"; date: "2009-09"; volume: "11"; issue: 
            "9"; pages: "1081-92"
COMMENT     Sequence Label: pSG5 N-HA SADBs shRNA resistant
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     92..111
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     139..158
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        139..157
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             177..203
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tggtttgaga tgaggataaa atactctgag tccaaaccgg gcccctctgc taaccatgtt
       61 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
      121 cattttggca aagaattgta atacgactca ctatagggcg aattgatatc accatgtacc
      181 cgtacgatgt cccggattac gcgaattcgg accagggcac catgtcgtcc gggtccaagg
      241 aaggtggcgg gggctccccc gcctaccacc tcccacgccc acacccacac ccaccccagc
      301 acgcccaata tgtgggcccc tatcgcctgg agaagacgct gggcaaagga cagacaggtc
      361 tagttaaact tggagtccac tgcatcacgg gtcagaaggt cgctgtcaag atcgtgaaca
      421 gggagaagct ttccgaatcg gtgctgatga aggtggagag ggaaatcgcc atcctgaagc
      481 tcattgaaca cccgcacgtg ctcaagctcc acgacgtcta cgagaacaag aaatatttat
      541 acttggttct tgagcacgtt tctggtggtg agctgttcga ctacctggta aaaaaaggga
      601 gactgacacc caaggaggcc cggaagttct tccgccagat cgtgtcagca ctggacttct
      661 gccatagcta ctccatctgt cacagagact tgaagccaga gaacctgctg ttggatgaga
      721 aaaacaacat ccgcatcgca gactttggta tggcgtccct gcaagtgggg gacagcctcc
      781 tggagaccag ctgcgggtcc ccccattacg catgtccaga ggtgatcaag ggggaaaagt
      841 atgatggccg ccgggcagac atgtggagct gtggagtcat cctatttgcc ctgcttgtgg
      901 gggcactgcc cttcgatgac gacaacctgc gccagctact ggagaaggtg aaacgtgggg
      961 tcttccacat gcctcacttc atccctccag actgccagag cctcctgaga gggatgattg
     1021 aagtggagcc cgagaaaagg ctcagtctgg agcaaattca gaaacatcct tggtatctgg
//
