LOCUS       sequence-117963-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express EGFP from the 
            CAG promoter under intersectional control by Flp and Cre 
            recombinases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..79)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(90..123)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      195..204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(201..203,204..206,207..719)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(246..267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(507..526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatgat cttcgataac ttcgtatagg
       61 atactttata cgaagttatt gcgattcgga taacttcgta tagcatacat tatacgaagt
      121 tatcaaggcc ggccaggttg gcgcgccaac tagctagcta tcaaggttag tacgttccaa
      181 gcttggggga attcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      241 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      301 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      361 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
      421 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      481 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      541 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      601 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      661 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
      721 a
//
