LOCUS       sequence-117966-         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express EGFP from the 
            CAG promoter under intersectional control by Flp and Cre 
            recombinases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    76..109
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    120..153
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(173..206)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    366..414
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     439..458
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 tggacgagct gtacaagtaa gaattccttg ggccccctcg aaggtacgtt gcgttattta
       61 aatttggcgc gccaaataac ttcgtataaa gtatcctata cgaagttatt gcgattcgga
      121 taacttcgta taatgtatgc tatacgaagt tatcgacgtc gaaaatctcg aggaagttcc
      181 tatactttct agagaatagg aacttccacg gcctgccgcc ccgacgttgg ctgcgagccc
      241 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
      301 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
      361 tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgtcatag
      421 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc cctactcaat
      481 tagctgaggc agaaggacag ggaagggagc agtggttcac gcctgtaatc ccagcaattt
      541 gggaggccaa ggtgggtaga tcacctgaga ttaggagttg gagaccagcc tggccaatat
      601 ggtgaaaccc cgtctctacc aaaaa
//
