LOCUS       sequence-117968-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express the 
            ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under 
            intersectional control by Flp and Cre recombinases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(41..74)
                     /label=loxJTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcgactagtg ataacttcgt atagcataca
       61 ttatagcaat ttatttggcg cgccatttaa atcctggccg gccttgccac catggattat
      121 ggaggcgccc tgagtgccgt tgggcgcgag ctgctatttg taacgaaccc agtagtcgtc
      181 aatggctctg tacttgtgcc tgaggaccag tgttactgcg cgggctggat tgagtcgcgt
      241 ggcacaaacg gtgcccaaac ggcgtcgaac gtgctgcaat ggcttgctgc tggcttctcc
      301 atcctactgc ttatgtttta cgcctaccaa acatggaagt caacctgcgg ctgggaggag
      361 atctatgtgt gcgctatcga gatggtcaag gtgattctcg agttcttctt cgagtttaag
      421 aacccgtcca tgctgtatct agccacaggc caccgcgtcc agtggttgcg ttacgccgag
      481 tggcttctca cctgcccggt cattctcatt cgcctgtcaa acctgacggg cttgtccaac
      541 gactacagca ggcgcaccat gggtctgctt gtgtctgata ttggcacaat tgtgtggggc
      601 gccacttccg ccatggccac cggatacgtc aaggtcatct tcttctgcct gggtctgtgt
      661 tatggtgcta acacgttctt tcacgctgcc aaggcctaca tcgagg
//
