LOCUS       sequence-117976-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human RAD21 along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_RAD21_1
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             55..120
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             127..147
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catggactat
       61 aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga tgacgataag
      121 atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag
      181 tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag
      241 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
      301 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
      361 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
      421 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
      481 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
      541 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
      601 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
      661 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
      721 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
      781 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctgg
//
