LOCUS       sequence-117991-        1803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSG5 containing N-terminally RFP tagged mouse SADBs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Alvarado-Kristensson M, Rodriguez MJ, Silio V, Valpuesta JM, Carrera
            AC
  TITLE     SADB phosphorylation of gamma-tubulin regulates centrosome 
            duplication.
  JOURNAL   Nat Cell Biol. 2009 Sep;11(9):1081-92. doi: 10.1038/ncb1921. Epub 
            2009 Aug 2.
  PUBMED    19648910
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1921"; 
            journalName: "Nat Cell Biol"; date: "2009-09"; volume: "11"; issue: 
            "9"; pages: "1081-92"
COMMENT     Sequence Label: pSG5 N-RFP SADBs
FEATURES             Location/Qualifiers
     source          1..1803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..684
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TG"
     CDS             13..684
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TG"
     primer_bind     complement(143..161)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     411..430
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     605..628
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gcggatatca ccatggcctc ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg
       61 cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      121 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
      181 gcctgggaca tcctgtcccc tcagttccag tacggctcca aggcctacgt gaagcacccc
      241 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      301 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
      361 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
      421 cagaagaaga ccatgggctg ggaggcctcc accgagcgga tgtaccccga ggacggcgcc
      481 ctgaagggcg agatcaagat gaggctgaag ctgaaggacg gcggccacta cgacgccgag
      541 gtcaagacca cctacatggc caagaagccc gtgcagctgc ccggcgccta caagaccgac
      601 atcaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
      661 gccgagggcc gccactccac cggcggcccg aattcgggac caggcaccat gtcgtccggg
      721 tccaaggaag gtggcggggg ctcccccgcc taccacctcc cacacccaca cccacaccca
      781 ccccagcacg cccaatatgt gggcccctat cgcctggaga agacgctggg caaaggacag
      841 acaggtctag ttaaacttgg agtccactgc atcacgggtc agaaggtcgc tgtcaagatc
      901 gtgaacaggg agaagctgtc ggaatcggtg ctgatgaagg tggagaggga aatcgccatc
      961 ctgaagctca ttgaacaccc gcacgtgctc aagctccacg acgtctacga gaacaagaaa
     1021 tatttatact tggttcttga gcacgtttct ggtggtgagc tgttcgacta cctggtaaaa
     1081 aaagggagac tgacacccaa ggaggcccgg aagttcttcc gccagatcgt gtcagcactg
     1141 gacttctgcc atagctactc catctgtcac agagacttga agccagagaa cctgctgttg
     1201 gatgagaaaa acaacatccg catcgcagac tttggtatgg cgtccctgca agtgggggac
     1261 agcctcctgg agaccagctg cgggtccccc cattacgcat gtccagaggt gatcaagggg
     1321 gaaaagtatg atggccgccg ggcagacatg tggagctgtg gagtcatcct atttgccctg
     1381 cttgtggggg cactgccctt cgatgacgac aacctgcgcc agctactgga gaaggtgaaa
     1441 cgtggggtct tccacatgcc tcacttcatc cctccagact gccagagcct cctgagaggg
     1501 atgattgaag tggagcccga gaaaaggctc agtctggagc aaattcagaa acatccttgg
     1561 tatctgggcg ggaaacacga accagaccct tgcctggagc cagccccagg ccgcagagta
     1621 gccatgcgta gcctgccttc caatggcgag ctggaccctg acgttctgga aagcatggcg
     1681 tctctgggct gcttcagaga ccgcgagcgg ctgcacagag aactgcgaag cgaggagtaa
     1741 gagctcccaa aatccttttc accggcgccc cacttcattg aatgggttgt ggcaggatcc
     1801 gcg
//
