LOCUS       sequence-117997-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl14 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL14-GFP
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(684..708)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(740..759)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(741..759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(804..855)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(833..853)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attcacaaga attgggacaa ctccagtgaa aagttcttct cctttgctag ccattctaga
       61 tcgaaccact ttgtacaaga aagctgggtc gttctgcttg aagaacgcca aagtgtctcc
      121 tcttgatttc atgtggctct tcacaaatcc agttaatttc ctgaacccct gggccagccc
      181 ctcccctgtg agggcacagc agggttgcac ataccagttc cggtcactgc aaagcttctt
      241 cactttgaac attctggtga tgtcctcagc agtcagagct ccaggcatgt cttgtttgtt
      301 ggctaataga acaacaggca catttttaat gtgttcattc ttcaaaatgt gctcaaactg
      361 tctctgagac tcttccagtc gctgtttgtc tgtactgtcc acaacataca ccagcccatc
      421 ggtgttctca cagtaacagc cccaaacagt tctcattttt tcctgtcctc caacatccca
      481 gactgtgagt gaaaaattcc tttccaactc gatcatttcc acattgaaac ctattgtagg
      541 gatggtggta atatccttag caagctttaa tttataaagg agagtagact tcccagctga
      601 gtcaagtccc aaaagaagaa cttgggcttg tttggtttgc ggatttttag aacccagcga
      661 acccatggtt ctatctcctt ctaagcctgc ttttttgtac aaacttgttt gatcaagctt
      721 aactagccag cttgggtctc cctatagtga gtcgtattaa tttcgataag ccagtaagca
      781 gtgggttctc tagttagcca gagagctctg cttatataga cctcccaccg tacacgccta
      841 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccggttgatt
      901 ttggtgccaa aacaaactcc cattgacgtc aa
//
