LOCUS       sequence-118000-         569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of murine PDGFRbeta tagged with GFP under the control of 
            EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Nielsen BS, Malinda RR, Schmid FM, Pedersen SF, Christensen ST, 
            Pedersen LB
  TITLE     PDGFRbeta and oncogenic mutant PDGFRalpha D842V promote disassembly 
            of primary cilia through a PLCgamma- and AURKA-dependent mechanism.
  JOURNAL   J Cell Sci. 2015 Oct 1;128(19):3543-9. doi: 10.1242/jcs.173559. Epub
            2015 Aug 19.
  PUBMED    26290382
REFERENCE   2  (bases 1 to 569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.173559"; journalName: "J Cell Sci"; date: "2015-10-1- 
            1"; volume: "128"; issue: "19"; pages: "3543-9"
COMMENT     Sequence Label: pcDNA5FRT-EF-Pdgfrbeta-EGFPN
FEATURES             Location/Qualifiers
     source          1..569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1..18)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7..231
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(364..383)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    515..562
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
       61 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      121 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
      181 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
      241 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
      301 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      361 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      421 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      481 aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt
      541 ctctagaaag tataggaact tccttggcc
//
