LOCUS       sequence-118002-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl11 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL11-GFP
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(696..720)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(752..771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(753..771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(816..867)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(845..865)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attcacaaga attgggacaa ctccagtgaa aagttcttct cctttgctag ccattctaga
       61 tcgaaccact ttgtacaaga aagctgggtc agatctcttg ctgtctccgc gctcagcccc
      121 atgggctctc gcctgcagac acatgcagct gcgagatttc aggaggctcc acaggctctg
      181 cagggcctcg ggcagcccct ccccagtgag ggcactgcag ccccggagct cccagcggtg
      241 gtcctggaat ctctctagac tcagcctgtt tctgatctta agcagcggaa gtgcatcagg
      301 tgcctcctgc ttgttggcca gcaccaagaa ggggacgcca gccatgttgg ggtcgttcag
      361 gacttctgtg agctcagccg ccgactcggg taagcgggct tcatctgtgc tgtccagcac
      421 gtacacgagg atatctgtgc cttccagata gtccttccag ctggctctga gcggggcctg
      481 ccccccaacg tcccagagag tcagtgacac gtgcccagga gctttcagag gctccacgtt
      541 gaaaccaaca gtgggcaggg tctccaccag ctggtggccc ttcagcttgt aaaggagcgt
      601 ggtcttgccc gccgagtcca ggcccatcat caccacctgg gcttccgcct tgtgacctct
      661 ggaattcaca gaacccatgg ttctatctcc ttctaagcct gcttttttgt acaaacttgt
      721 ttgatcaagc ttaactagcc agcttgggtc tccctatagt gagtcgtatt aatttcgata
      781 agccagtaag cagtgggttc tctagttagc cagagagctc tgcttatata gacctcccac
      841 cgtacacgcc taccgcccat ttgcgtcaat ggg
//
