LOCUS       sequence-118004-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArfrp with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARFRP1-GFP
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(707..731)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(763..782)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(764..782)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(827..926)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(856..876)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acaagaattg ggacaactcc agtgaaaagt tcttctcctt tgctagccat tctagatcga
       61 accactttgt acaagaaagc tgggtccgtg atgtccctct gccgcggcgg ccggtgcaca
      121 ttccgcacga cacacttcac catccactcg atgccctcgc gcaccccttt gcctgtgagg
      181 gccgagcagg cctgggtcag gcaatcgcgc ctgccgatct tgctggtgca gtcgctgaag
      241 gccgtcttga tgtcagggat tgagaggcac gtctccacat cctgcttgtt ggccagcacc
      301 aagacgggga caccgcacag cgcctcgctg gtcaccacct tctcaaacgc ctgcttggac
      361 tcagccagcc tctcctcgtc ggtggagtca atgacgtaga tgacgccgtg acactccgca
      421 taatacttgt cccacaaaga ctgcagctct tcctgccctc ctaagtccca gaacatgagc
      481 cgagcctttc ccacatccac agtgccgatg tttaggccca cggtggtggt gattttggat
      541 agactcatcc ccttgtagtt cttgttaaat cgggttttcg actgctccag gaaggtcgtc
      601 ttcccagcat tgtccaggcc caggatcagg atgcagtact cgtccttctg aaacatgtac
      661 ttgtacaagc ccgacagcag cgtgtacatg gttctatctc cttctaagcc tgcttttttg
      721 tacaaacttg tttgatcaag cttaactagc cagcttgggt ctccctatag tgagtcgtat
      781 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
      841 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      901 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggg
//
