LOCUS       sequence-118005-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hSar1 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-SAR1-GFP
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(695..719)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(751..770)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(752..770)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(815..914)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(844..864)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcgtcaata tactgggaga gccagcggaa accctcgccg
      121 taaccttgcc tcttgagcac actgcacatg aacacttcca tggggcgagc attcagctcc
      181 ttcagggtca cattcccctt tcctgtggtc tgtccataaa gcccaaatat ctcacggagt
      241 ttttcttcac tgattgcatc tgttctgtca attttgttac ccaagataag gattggcaca
      301 ttggatattg tttcatcagt cattaaagca ttaagctcaa ctttggattc cacgaggcga
      361 gaatgatctg cacagtccac cagaaagaca atcccattaa ttgctgggag ataatttttc
      421 caaacgcgac gtgcttgctc gtgcccacca agatcaaaag ttgtaaaggt cattccagca
      481 attgttagct cttctgatgt cggatgtagt gttggaacat gttggcccaa tctgtcatct
      541 ttgagcatgt gaagaagagt ggttttgcct gcattatcca aacctaagaa tacaagtttt
      601 ccagatttct tgtacagtcc taggaactgg agcacactgc tgaagccatt gtagatccac
      661 tcaaagatga aagacatggt tctatctcct tctaagcctg cttttttgta caaacttgtt
      721 tgatcaagct taactagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa
      781 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc
      841 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
      901 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
      961 atccccgt
//
