LOCUS       sequence-118006-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hSara with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-SARA-GFP
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(695..719)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(751..770)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(752..770)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(815..914)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(844..864)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcatcaatg tactgtgcca tccagcggaa gccttctccg
      121 taaccttgtc ttttgagcac actacacatg aaaacttcta agggtcgggc attcagttct
      181 ttcagagata tactcccctt tcctgttgtc tgaccatata aaccaaacat ctctcgcaac
      241 ctctcttcac tgatggcttc aggtctgtcg atcttattcc caagaatcag tataggcaca
      301 ttagcaatgg tttcatctgt cattagtgaa tcaagttctt cttttgactc taacagcctt
      361 tcgtggtctg cacaatccac cagaaataca atgccattga tagcaggaag gtagtttttc
      421 cacactcttc gagcttgaac atgtccaccc agatcaaaag ttgtaaacgt catgccagca
      481 atggtcagtt cttcggaagt gggatgtaat gttgggacat gttgtccaag tctgtcatct
      541 tttagcatgt gtagcaatgt tgtttttcct gcattatcca atccaagaaa taccagttta
      601 ccagttttct tatataatcc taaaaactgt agcacactgc tgaaaccact gtaaatccaa
      661 tcaaatatga aggacatggt tctatctcct tctaagcctg cttttttgta caaacttgtt
      721 tgatcaagct taactagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa
      781 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc
      841 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
      901 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
      961 atccccgtga gtcaaaccgc tatcacgc
//
