LOCUS       sequence-118009-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArf5 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARF5-V5-His6
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(61..102)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    122..146
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(705..729)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    770..794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(794..813)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(795..813)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(897..916)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcaaac tcaatggtga tggtgatgat gaccggtacg
       61 cgtagaatcg agaccgagga gagggttagg gataggctta ccttcaagct tcgaattgat
      121 caccactttg tacaagaaag ctgggtcgcg ctttgacagc tcgtgggaca gccagtccag
      181 accatcgtac agacctgtgc cttgggtggc acaggtggcc tggacatacc acgtgcggct
      241 gcgtaagtgc tgtagcccca gcttgtcagt cagctcgctc acgggcatgg cgttgggcat
      301 gtcctgcttg ttggcaaata ccagcagcac tgcatcccgc agctcgtcct cctgcagcat
      361 cttctggagt tcatcagcag attcttggac ccgctcccgg tcattactgt ccaccacaaa
      421 gatgaggccc tgagtgttct ggaagtagtg ccgccacaga ggccgaatct tgtcctggcc
      481 tcccacgtcc cagactgtga aacagatgtt cttatattcc actgtttcta cattgaagcc
      541 tatggttggg atggtggtga caatctcccc caacttcagt ttgtacagga ttgtggtctt
      601 gccagccgca tccaagccaa ccatgagaat ccgcatctgc ttcttcccga agatccgcga
      661 aaagagcgcg gacacggtga ggcccatggt tctatctcct tctaagcctg cttttttgta
      721 caaacttgtg atattccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      781 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
      841 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      901 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcggg
//
