LOCUS       sequence-118010-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArf6 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARF6-V5-His6
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(62..103)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    123..147
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(691..715)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    756..780
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(780..799)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(781..799)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(883..902)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatcaaa ctcaatggtg atggtgatga tgaccggtac
       61 gcgtagaatc gagaccgagg agagggttag ggataggctt accttcaagc ttcgaattga
      121 tcaccacttt gtacaagaaa gctgggtcag atttgtagtt agaggttaac catgtgagcc
      181 cctcatagag tccgtcccct gaggtggcac aggagggctg cacataccag ttcctgtccc
      241 gaatccgggt caggcccagt ttctcctgga tctcgtgggg tttcatggca tcgggcaggt
      301 cctgcttgtt ggcgaagatg aggattatgg cgtccctcat ctcccggtca ttgataatgc
      361 ggtgcagctc ctggcgagcc tcatcgatgc ggtcgcggtc ggcgcagtcc actacgaaga
      421 tgagaccttg ggtcccagtg tagtaatgcc gccagagcgg ccggatcttg tcctggccgc
      481 ccacatccca tacgttgaac ttgacatttt tgtaagtcac cgtctccacg ttgaaaccca
      541 cagtgggaat ggtggtcacc gactggccca gcttcaactt gtacaggatt gttgtcttgc
      601 cggccgcgtc caggcccaac atgaggatcc gcatttcctt gttcccgaag attttggata
      661 gcaccttccc catggttcta tctccttcta agcctgcttt tttgtacaaa cttgtgatat
      721 tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc tcacaattcc
      781 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac gccggacgca
      841 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
      901 ccgatgggga aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
      961 atggtggcag gccccgtggc cgggg
//
