LOCUS       sequence-118012-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArl1 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARL1-V5-His6
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    118..142
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(704..728)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    769..793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(793..812)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(794..812)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(896..915)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggctttgtt agcagccgga tcaaactcaa tggtgatggt gatgatgacc ggtacgcgta
       61 gaatcgagac cgaggagagg gttagggata ggcttacctt caagcttcga attgatcacc
      121 actttgtaca agaaagctgg gtcctgtctg ctttttaatg tttcaactaa ccattccatt
      181 gcctcatcaa ggccggtgcc tttggttgct gacgttttga atatctgcca ttttcggtcc
      241 ttcaaggcag gtaacccaag tgaatttgcc atctctgagg aagtcatggc ctgttccatg
      301 tcctgtttat ttgcaaacac cactaaaatg gcttttctca gctcttcttc ctccaacatg
      361 gcaactaact ctgatttgga aatgccaatt cggtctcggt cacaactgtc tactacataa
      421 atgactgcat ctgtgtttga atagtaacat ctccaatatg gcctgatact tgtctgtcct
      481 cctaaatccc agacttggaa tttaaggttt ttgtacgtca ccgtctctac attaaatcca
      541 atggtaggta tagtagtaac aacttctccc acttgtaatc tgtacaaaat tgtggttttt
      601 cctgctccat ctaatcccaa aattaaaatt ctcatttccc gagttccaaa cagactggaa
      661 aatatacttg agaaaaagcc acccatggtt ctatctcctt ctaagcctgc ttttttgtac
      721 aaacttgtga tattccttct taaagttaaa caaaattatt tctagagggg aattgttatc
      781 cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc
      841 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
      901 atcgccgaca tcaccgatgg g
//
