LOCUS       sequence-118014-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArl2 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARL2-V5-His6
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(56..97)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    117..141
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(712..736)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    777..801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(801..820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(802..820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggctttgtta gcagccggat caaactcaat ggtgatggtg atgatgaccg gtacgcgtag
       61 aatcgagacc gaggagaggg ttagggatag gcttaccttc aagcttcgaa ttgatcacca
      121 ctttgtacaa gaaagctggg tcgtcagctg tgaaaatgcg gctggaaatg tcatccagga
      181 gccagtcgat gcccggcagc aggttctccc cggtgacggc gctgcagccc tggatgcacc
      241 agtggtggct gcggatggag tccagctcca gggcctcgcg gatggcgtta gaggacagtg
      301 ctccaggcag gtcctgctta ttagcaaaga tgaggagggt tgctccggcc aggcgctcct
      361 ccaccagcag gctctggagc tcccgctggc agtcctgcat gcgctggcgg tctgcgctgt
      421 ccactaccca gatgaggcca tcggtgctct caaagtagtt ccgccagtag gaccgcaggg
      481 acttctggcc acccacatcc cagatgttca gcttgaatcc tcggtgctcc agggtcttga
      541 tgttgaagcc cagcgttggg gagatggtgt cgatgtcctc cccattgaac ttcttcagga
      601 tggttgtctt tccagcattg tccaggccaa gcatgagcag tcgcagctcc cgctctttct
      661 gcttcatctt cttcagaatg gtcaggagcc ccatggttct atctccttct aagcctgctt
      721 ttttgtacaa acttgtgata ttccttctta aagttaaaca aaattatttc tagaggggaa
      781 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatct
      841 cgatcctcta cgccggacgc atcgtggccg gcatcaccg
//
