LOCUS       sequence-118022-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArl4 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARL4-V5-His6
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    118..142
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(761..785)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    826..850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(850..869)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(851..869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggctttgtt agcagccgga tcaaactcaa tggtgatggt gatgatgacc ggtacgcgta
       61 gaatcgagac cgaggagagg gttagggata ggcttacctt caagcttcga attgatcacc
      121 actttgtaca agaaagctgg gtctcttttc tttttctgtt gccgcaacat ttttcttctt
      181 ttaatgatca tatcatgtag tttctcaagt ccttccttta ggccatctcc tatgattgca
      241 caggtaggct gcaaatgcca aggagttgat gagctcagtt cacccattgc taacaatttc
      301 tcaatttctg aaagtgacaa tgagttcctc aaatcttgtt tgttagcaac tataagtaca
      361 gggactccct gattttctga tatcctagtt attttgtgaa gttcagtttt ggcttcttcc
      421 atcctttcga catcaacaga gtccacaaca aatacaatgc catctgtgca tctggtatat
      481 gacttccaca gtggccttaa tttctcctga ccacctacat cccagaagtg aaaagtgact
      541 gttttagaat ttcccaaggt taccttaatt ttctcagtgt taaatccttt ggtaggtacg
      601 gtatttacaa attcattgaa ctgcagcctg tataagacag ttgtctttcc agcacagtcc
      661 aaacccagaa taacaatgtg gaaagactga aatgaaggca ggttggacag gatagaagtc
      721 tggtctgaca gcccattccc catggttcta tctccttcta agcctgcttt tttgtacaaa
      781 cttgtgatat tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc
      841 tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
      901 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      961 gccgacatca c
//
