LOCUS       sequence-118053-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArl5 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARL5A-V5-His6
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(60..101)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    121..145
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(701..725)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    766..790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(790..809)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(791..809)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcaaact caatggtgat ggtgatgatg accggtacgc
       61 gtagaatcga gaccgaggag agggttaggg ataggcttac cttcaagctt cgaattgatc
      121 accactttgt acaagaaagc tgggtctcta atcttaagtc gtgacatcat ccattcaagt
      181 ccttggcaca atccctcgcc agttagagca cagcatgcct ggatatgcca ctggtgatct
      241 ttaatagaag ttagcttcaa aaactgggag atttctgcta cagtcatgca ttctttaaca
      301 tcttgtttat tagcaaaaat cagcaatcca gcttttctta ggtcctcatg cgctaacatt
      361 ttatagagtt cttctctagt tacagaaatc ctctctctgt ctgtactgtc cacaacaact
      421 attacaaact ctgtgttagt atagtaagtg ttccaggaag aacgaagaga ttcttggcca
      481 ccaatatccc acattaggaa acgtgtatta ttaatcacta tctcttctac attacttcct
      541 attgtaggag atgtatgtac aacttcattc atagaaaatt ggtaaagaat ggtagttttc
      601 cctgcattat ccaacccaac aatgataact ttgtgctcct ggtgattgaa cagtctccat
      661 attctagtga agagaattcc catggttcta tctccttcta agcctgcttt tttgtacaaa
      721 cttgtgatat tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc
      781 tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
      841 gccggacgca tcgtggcccg gcat
//
