LOCUS       sequence-118054-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArl6 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARL6-V5-His6
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    118..142
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(719..743)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    784..808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(808..827)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(809..827)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggctttgtt agcagccgga tcaaactcaa tggtgatggt gatgatgacc ggtacgcgta
       61 gaatcgagac cgaggagagg gttagggata ggcttacctt caagcttcga attgatcacc
      121 actttgtaca agaaagctgg gtctgtcttc acagtctgga tctgatcttg aagccagtct
      181 acaccttctt gcaagccttc tccttttatg gcatcactag cacaaatatg ccagggttta
      241 tctttgatgt tctctaaaca cagcaactga gacactttta cagatgtcac tgcatctcta
      301 agatccattt tatttgcaaa gaataagatt ggaattcgac ggtgtttaat atctggatga
      361 ttcagaagag tatcgagttc ttctttggcc acaaccattc ttaatctatc actactatca
      421 atgacaaaaa taatagcttg gccttcttta taatagtgtt cccagagatt tctgtatctt
      481 ccttgacctg acatgtcaaa cactgtaaat gacaaactgg atgatttgaa tttctctatg
      541 ctgaatccta ttgttggaag gatattttga gattgagcat ttgaaggttt aagtttgtta
      601 atgatcgtcg ttttgccact attatctagc ccaaggcaca aaacatgaac ctccttcttc
      661 ttcaggccaa gcaagactga aagtctgtct agcaatccca tggttctatc tccttctaag
      721 cctgcttttt tgtacaaact tgtgatattc cttcttaaag ttaaacaaaa ttatttctag
      781 aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc
      841 gagatctcga tcctctacgc cggaacgcat cgtgggccgg catcaccggc gccacaggtg
      901 cgggttgctg gcgcctatat cgcccaacat c
//
