LOCUS       sequence-118055-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mNanog reporter plasmid (expresses YNL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pRZ-mNanog-YNL
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(34..55)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(190..702,703..705,706..708))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     383..402
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     642..663
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggggtcgtac accttggtac ccccggcggc ggtcacgaac tccagcagga ccatgtgatc
       61 gcgcttctcg ttggggtctt tgctcagctt ggactggtag ctcaggtagt ggttgtcggg
      121 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      181 gccgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt tcttctgctt
      241 gtcggcggtg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
      301 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      361 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
      421 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
      481 ggggtagcgg gcgaagcact gcaggccgta gcccagggtg gtcacgaggg tgggccaggg
      541 cacgggcagc ttgccggtgg tgcagatcag cttcagggtc agcttgccgt aggtggcatc
      601 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      661 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggtggatcc
      721 gcagccttcc cacagaaaga gcaagacacc aaccaaatca gcctatctga aggccaacgg
      781 ctcaaggcga tagatttaaa gggtaggtcc tgctcctcc
//
