LOCUS       sequence-118060-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A transformation-associated recombination (TAR) based genomic 
            capture and expression vector for antibiotic production in B. 
            subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Li Y, Li Z, Yamanaka K, Xu Y, Zhang W, Vlamakis H, Kolter R, Moore 
            BS, Qian PY
  TITLE     Directed natural product biosynthesis gene cluster capture and 
            expression in the model bacterium Bacillus subtilis.
  JOURNAL   Sci Rep. 2015 Mar 24;5:9383. doi: 10.1038/srep09383.
  PUBMED    25807046
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-03-24"; volume: "5:9383. doi"; pages: " 
            10.1038/srep09383"
COMMENT     Sequence Label: pCAPB02
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(192..296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     364..382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
       61 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      301 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
      361 aaaaataggc gtatcacgag gccctttcgt cttcaagaat taacaaaatt ctccagtctt
      421 cacatcggtt tgaaaggagg aagcggaaga atgaagtaag agggattttt gactccgaag
      481 taagtcttca aaaaatcaaa taaggagtgt caagaatgtt tgcaaaacga ttcaaaacct
      541 ctttactgcc gttattcgct ggatttttat tgctgtttca tttggttctg gcaggaccgg
      601 cggctgcgag tgctgaaacg gcgaacaaat cgaatgagct tacagcaccg tcgatcaaaa
      661 gcggaaccat tcttcatgca tggaattggt cgttcaatac gttaaaacac aatatgaagg
      721 atattcatga tgcaggatat acagccattc agacatctcc gattaaccaa gtaaaggaag
      781 ggaatcaagg agataaaagc atgtcgaact ggtactggct gtatcagccg acatcgtatc
      841 aaattggcaa ccgttactta ggtactgaac aagaa
//
