LOCUS       sequence-118067-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Blue light dependent photoswitching system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Kawano F, Suzuki H, Furuya A, Sato M
  TITLE     Engineered pairs of distinct photoswitches for optogenetic control 
            of cellular proteins.
  JOURNAL   Nat Commun. 2015 Feb 24;6:6256. doi: 10.1038/ncomms7256.
  PUBMED    25708714
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-02-24"; volume: "6:6256. doi"; pages: " 
            10.1038/ncomms7256"
COMMENT     Sequence Label: pcDNA3.1_pMagFast2(3x)-iRFP
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             114..563
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMagFast2
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with fast switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDTSCALVLCDLKQK
                     DTPVVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagctta agctggctag cgccgccacc atgcacactc
      121 tttacgcccc tggaggatac gacattatgg gatatttgcg gcagattagg aaccgcccaa
      181 accctcaggt cgaactgggg cctgtggaca cgtcatgtgc cctggtcctg tgcgatctga
      241 agcaaaagga cactccggtg gtctacgcct cggaagcctt cttgtatatg accggataca
      301 gcaatgcaga ggtgctcggc aggaactgca gattcctgca gtcccccgac gggatggtga
      361 aaccaaagtc gactcgcaaa tatgtggact cgaacacgat caacaccatg cggaaggcca
      421 tcgaccggaa cgccgaggtc caggtggagg tggtcaactt taagaagaac ggccagcggt
      481 tcgtgaactt tctgaccatg attccggtcc gggatgaaac cggagagtac agatactcca
      541 tgggattcca gtgcgaaacc gaaggttccg gcggctcggg cggatcaggg ggaagcggac
      601 acacactcta cgccccggga ggctacgata tcatgggcta cctgaggcag atccggaaca
      661 gaccgaaccc ccaagtggaa ctcggccccg tggacacttc ttgcgctctg gtgctgtgcg
      721 acctgaagca gaaggatacc cccgtggtgt acgcatccga agcgttcctc tacatgaccg
      781 g
//
