LOCUS       sequence-118102-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TagBFP-mEmerald-FRET
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..761
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattca
       61 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
      121 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
      181 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
      241 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
      301 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
      361 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
      421 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
      481 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
      541 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat
      601 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
      661 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
      721 gatactgcga cctccctagc aaactggggc acaagcttaa ttccggactc agatcccc
//
