LOCUS       sequence-118103-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TagBFP-mEmerald-FRET
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(127..378)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(169..190)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     518..537
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcta gagtcgcggc
      121 cgcttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      181 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agcttggact gggtgctcag
      241 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      301 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg gtcttgaagt tcaccttgat
      361 gccgttcttc tgcttgtcgg cggtgatata gaccttgtgg ctgttgtagt tgtactccag
      421 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      481 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      541 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
      601 ctgcttcatg tggtcggggt agcgggcgaa gcactgcacg ccgtaggtca aggtggtcac
      661 gagggtgggc cagggcacgg gca
//
