LOCUS       sequence-118119-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Blue light dependent photoswitching system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Kawano F, Suzuki H, Furuya A, Sato M
  TITLE     Engineered pairs of distinct photoswitches for optogenetic control 
            of cellular proteins.
  JOURNAL   Nat Commun. 2015 Feb 24;6:6256. doi: 10.1038/ncomms7256.
  PUBMED    25708714
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-02-24"; volume: "6:6256. doi"; pages: " 
            10.1038/ncomms7256"
COMMENT     Sequence Label: pcDNA3.1_nMagHigh1-EGFP-CAAX
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             117..566
                     /codon_start=1
                     /product="""negative Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=nMagHigh1
                     /note="rapidly heterodimerizes with pMag or pMagFast2 upon 
                     blue light irradiation"
                     /translation="HTLYAPGGYDIMGYLDQIGNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTIRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTIIPVRDETGEYRYSMGFQCETE"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagctta agctggctag cgccgccacc atgggacaca
      121 ctctttacgc ccctggagga tacgacatta tgggatattt ggatcagatt gggaaccgcc
      181 caaaccctca ggtcgaactg gggcctgtgg acacgtcatg tgccctgatc ctgtgcgatc
      241 tgaagcaaaa ggacactccg atcgtctacg cctcggaagc cttcttgtat atgaccggat
      301 acagcaatgc agaggtgctc ggcaggaact gcagattcct gcagtccccc gacgggatgg
      361 tgaaaccaaa gtcgactcgc aaatatgtgg actcgaacac gatcaacacc atccggaagg
      421 ccatcgaccg gaacgccgag gtccaggtgg aggtggtcaa ctttaagaag aacggccagc
      481 ggttcgtgaa ctttctgacc atcattccgg tccgggatga aaccggagag tacagatact
      541 ccatgggatt ccagtgcgaa accgaaggcg gctccggagg cagcggcggt tctggaggtt
      601 ccggtggcgg ctccatggtg tcgaagggag aggagctttt caccggcgtg gtgccgattc
      661 ttgtggaact ggacggcgac gtgaacggac ataagttcag cgtgtcagga gaaggagaag
      721 gggacgcgac ttacggaaag ctgaccctga agttcatctg taccaccgg
//
