LOCUS       sequence-118127-        2522 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2522)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..2522
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1697..1749
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1757..1766
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1763..1765,1766..1768,1769..2482)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1808..1829)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2069..2088)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2416..2437
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcacca tggacgacct cgatgcctta ctggcagacc tggaatccac
       61 cacctcgcat atctctaagc gaccggtgtt tctaacagag gaaacgcctt actcctaccc
      121 aactggaaac catacgtacc aggagattgc tgtgccaccg ccggtgcccc cgccgccttc
      181 cagtgaggcc ctaaatggca ctgtgattga ccccttagac cagtggcaac ccagcgtgtc
      241 cagatacggc caccagcaac ctcagtccca gtctccgata tacagctcta gtgccaaaag
      301 ctccagtgcc tctgttccta gagacgggct cagctcccct tctccccgtg ccagtgagga
      361 ggaacacgtg tacagcttcc caaacaagca gaagtctgca gaaccatctc ccacaatgac
      421 cagcacctcc ctgggcagca acctctcgga actggacagg cttctcctgg aactgaatgc
      481 tgttcaacat aatcccccca gtggcttctc agcagatgaa gtcagcagaa gcccatcact
      541 gcccaatgtg accggacctc actatgtcat cccagagagc agcagctctg caggagggaa
      601 ggctgcaccc cctacaaaag aaaaaccaaa gcggaatggt gggcgtggga ttgaagatgt
      661 gcgtcccagt gtggagagcc tgctggatga gctggagagc tctgtgccaa gcccagtccc
      721 tgcaatcact gtgagccaag gggaggtgag cagcccccag cgtgtcaacg ccagtcagca
      781 gcagacccgt atctctgctt cttcagctac acgagaactg gatgagctga tggcgtccct
      841 ctctgacttt aagttcatgg cacaggggaa agccggcggc agcagctccc caccatccac
      901 caccccaaag cctggcagtc agctggacac catgctggga agtctgcagt ctgacctgaa
      961 caaactgggg gtagcgacgg tcgccaaggg tgtgtgcgga gcctgcaaga agccgattgc
     1021 tgggcaggta gttacagcca tggggaagac ctggcaccct gagcacttcg tctgcaccca
     1081 ctgccaggag gagattggat cacggaactt ctttgagcgg gatgggcagc cctactgtga
     1141 gaaggactat cacaacctct tctctcctcg ctgctactac tgcaatgggc caatccttga
     1201 taaagtggtg acggctttgg acaggacgtg gcaccctgaa cactttttct gtgcccagtg
     1261 tggagttttc tttggacctg aaggatttca tgagaaggat ggcaaagcct attgccgcaa
     1321 ggactacttt gacatgtttg ctcccaagtg tggaggctgt gcccgggcta tcctggaaaa
     1381 ttacatttct gccttaaaca ccctgtggca ccctgagtgc tttgtctgtc gggaatgttt
     1441 cacaccgttc atcaacggca gcttcttcga gcacgacggg cagccctact gcgaggtgca
     1501 ttaccacgag cgccgcggct cgctgtgctc tggctgccag aagcccatca cgggacgctg
     1561 catcactgcc atgggcaaga aatttcaccc cgaacacttt gtctgtgcct tctgcctcaa
     1621 gcagctcaac aaaggaacct tcaaagaaca gaacgacaag ccctactgtc agaactgctt
     1681 tctcaaactc ttctgtctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
     1741 ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     1801 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1861 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
     1921 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc
     1981 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2041 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2101 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2161 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     2221 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     2281 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2341 cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa
     2401 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2461 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     2521 tt
//
