LOCUS       sequence-118128-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, 
            mVenus-tagged RhoC Q63L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Zawistowski JS, Sabouri-Ghomi M, Danuser G, Hahn KM, Hodgson L
  TITLE     A RhoC biosensor reveals differences in the activation kinetics of 
            RhoA and RhoC in migrating cells.
  JOURNAL   PLoS One. 2013 Nov 5;8(11):e79877. doi: 
            10.1371/journal.pone.0079877. eCollection 2013.
  PUBMED    24224016
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-11-5"; volume: "8(11):e79877. doi"; pages: " 
            10.1371/journal.pone.0079877. eCollection 2013"
COMMENT     Sequence Label: RhoC FLARE.sc mCer, mVenus - CA
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     113..134
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(411..662)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(453..474)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     802..821
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gagggggcag ggcagggcag ctgccggtgg tgcagatcaa cttcagggtc agctttgccg
       61 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
      121 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatt
      181 gcggccgcag atccaccttt agtagaacct tcaccagaac caggtttacc agaaccagaa
      241 gtagatccac ctttagtaga accttcacca gaaccaggtt taccagaacc agaagtagat
      301 ccacctttag tagaaccttc accagaacca ggtttaccag aaccagaagt agatccacct
      361 ttagtagaac cttcaccaga accaggttta ccagaaccag aagttaagct tttgtacagc
      421 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
      481 cgcttctcgt tggggtcttt gctcagcttg gactgggtgc tcaggtagtg gttgtcgggc
      541 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
      601 ctgccgtcct cgatgttgtg gcggatcttg aagttggcct tgatgccgtt cttctgcttg
      661 tcggcggtga tatagacgtt gtcgctgatg gcgttgtact ccagcttgtg ccccaggatg
      721 ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc
      781 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc
      841 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
      901 gggtagcggg cgaagcactg cacgccccag gtcagggtgg tcacgagggt gggccagggc
      961 acgggcagct
//
