LOCUS       sequence-118129-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A transformation-associated recombination (TAR) based genomic 
            capture and expression vector for antibiotic production in B. 
            subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Li Y, Li Z, Yamanaka K, Xu Y, Zhang W, Vlamakis H, Kolter R, Moore 
            BS, Qian PY
  TITLE     Directed natural product biosynthesis gene cluster capture and 
            expression in the model bacterium Bacillus subtilis.
  JOURNAL   Sci Rep. 2015 Mar 24;5:9383. doi: 10.1038/srep09383.
  PUBMED    25807046
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-03-24"; volume: "5:9383. doi"; pages: " 
            10.1038/srep09383"
COMMENT     Sequence Label: pCAPB02
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(20..38)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    79..582
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat
      121 gatggaataa tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt
      181 ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag
      241 gtttaaaaaa tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca
      301 gattaaatag atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt
      361 gtgtggtctt ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag
      421 agcaagataa aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa
      481 acaaaaacta ttttttcttt aatttctttt tttactttct atttttaatt tatatattta
      541 tattaaaaaa tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc
      601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acaccatgga
      661 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
      721 tccggtgccc tgaatgaact gcagaaagag ctggtagttg gcgcactgtt cgaagaactg
      781 ccgatgtcca gtaagattct tactatgctg gttgaaccgg atgctggtaa agctacttgg
      841 g
//
