LOCUS       sequence-118134-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, 
            mVenus-tagged RhoC Q63L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Zawistowski JS, Sabouri-Ghomi M, Danuser G, Hahn KM, Hodgson L
  TITLE     A RhoC biosensor reveals differences in the activation kinetics of 
            RhoA and RhoC in migrating cells.
  JOURNAL   PLoS One. 2013 Nov 5;8(11):e79877. doi: 
            10.1371/journal.pone.0079877. eCollection 2013.
  PUBMED    24224016
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-11-5"; volume: "8(11):e79877. doi"; pages: " 
            10.1371/journal.pone.0079877. eCollection 2013"
COMMENT     Sequence Label: RhoC FLARE.sc mCer, mVenus - CA
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..230
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     292..311
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     353..372
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        353..371
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    372..396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        412..521
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 gcgagtcgtt agtgaaccgt cagatcacta gatgctttat tgcggtagtt tatcacagtt
       61 aaattgctaa cgccagtctc gaacttaacg tgcagaagtt ggtcgtgagg cactgggcag
      121 gtaagtatcg ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
      181 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
      241 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
      301 tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgaa attaatacga
      361 ctcactatag gggaattgtg agcggataac aattccccgg agttaatccg ggacctttaa
      421 ttcaacccaa cacaatatat tatagttaaa taagaattat tatcaaatca tttgtatatt
      481 aattaaaata ctatactgta aattacattt tatttacaat caaaggagat ataccatggc
      541 gcgaggcctt ctggaagaac agtattttga attgacgcaa gaaagcaaga aagctgcttc
      601 aagaaataga caagagatta cagataaaga tcacactgtt agtcggcttg aagaagcaaa
      661 cagcatgcta accaaagata ttgaaatatt aagaagagag aatgaagagc taacagagaa
      721 aatgaagaag gcagaggaag aatataaact ggagaaggag gaggagatca gtaatcttaa
      781 ggctgc
//
