LOCUS       sequence-118135-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArl4c with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARL4C-V5-His6
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(56..97)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    117..141
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(736..760)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    801..825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(825..844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(826..844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(928..947)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggctttgtta gcagccggat caaactcaat ggtgatggtg atgatgaccg gtacgcgtag
       61 aatcgagacc gaggagaggg ttagggatag gcttaccttc aagcttcgaa ttgatcacca
      121 ctttgtacaa gaaagctggg tcccgcttct tcttctgctt gagggacttc ctgcgtttca
      181 ggatcatctc atagagcttg tccatgccct cggtgaggcc ctcgccgatg atggcgcacg
      241 ccggctggac gtgataggtg gtggccggga taagctcgtg cagcgccagc tgcttctcaa
      301 tctctgccac cggcagcgac ttgggcaggt cctgcttgtt ggcgatgacc agcagcggcg
      361 tgccctggtt ctcggcgaac ttggtcacct tgtgcagctc cgtcttggcc tcctccagcc
      421 ggtccacgtc caccgagtcc accacgtaga tgatgccgtc cgtgcagcgg ctgtaggact
      481 tccacagcgg ccgcagcttc tcctggccgc ccacgtccca gaagtggcag ctgatgccct
      541 tggccgtgcc gttgctcagc ttgatcttct cggtgttgaa gccgatggtg ggcaccgtgt
      601 tcacgaactc gttgaacttg agccggtaga gcaccgtggt cttgccggcc gagtccaagc
      661 ccaacatgac gatatgcagg gactggaagg ccgagatgtt agaggagatg ttgcccatgg
      721 ttctatctcc ttctaagcct gcttttttgt acaaacttgt gatattcctt cttaaagtta
      781 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
      841 attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
      901 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
      961 gggctcgcca cttcgggctc a
//
