LOCUS       sequence-118136-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArl8 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARL8A-V5-His6
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    118..142
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(719..743)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    784..808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(808..827)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(809..827)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(911..930)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggctttgtt agcagccgga tcaaactcaa tggtgatggt gatgatgacc ggtacgcgta
       61 gaatcgagac cgaggagagg gttagggata ggcttacctt caagcttcga attgatcacc
      121 actttgtaca agaaagctgg gtcgcttctc cgtgacttcg agtgttgaat aagccactgt
      181 agggtgatgt caatgttgtc cttttctttg caagagatgg agtagcagca gatctctcgg
      241 tcctggatgg cagacagatt cattttctca atcagctcct tctcatccaa tgctcccgga
      301 aggtctcgct tgttacccag gactaagacc gggatgccct gcagctgagg tttgtccagt
      361 aggttgtgga gctcgttctt agaggcctca atcttctcct ggtcagcagc atccaccatg
      421 tacacgatgg cgctcactcc tcggcagtag cgctcccaca tgctgcggaa acgcggctgt
      481 cccccaatgt cccagagctt gatagtcaca ttccctttgg tgattttgcg catgttgaaa
      541 cccacggtgg ggatcatgtc ctcgttgaac tgtcctgacg cgatcacgtt gacgaaggtg
      601 gtcttgcccg agtactgaag cccgaccagc gtgagctcca tctcctcctt ccagaatagg
      661 gccttgaacc agtccagcag cttgttgaac aaagcgatca tggttctatc tccttctaag
      721 cctgcttttt tgtacaaact tgtgatattc cttcttaaag ttaaacaaaa ttatttctag
      781 aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc
      841 gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
      901 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg cc
//
