LOCUS       sequence-118138-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArl8b with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARL8B-V5-His6
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    118..142
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(718..742)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    783..807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(807..826)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(808..826)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(910..929)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggctttgtt agcagccgga tcaaactcaa tggtgatggt gatgatgacc ggtacgcgta
       61 gaatcgagac cgaggagagg gttagggata ggcttacctt caagcttcga attgatcacc
      121 actttgtaca agaaagctgg gtcgcttctt ctagattttg aatgctgaat aagccactga
      181 agtgtgatat ctatattatc cttttctttg caagaaattg aatagcagca aatttctcta
      241 tcctgaatag cagacagatt cattttttca attagctgtt tctcatccaa ggcattagga
      301 agatctctct tgtttccaag cactagcact ggaattcctt gtaactgtgg tttatctaga
      361 agattatgta gctcatttcg ggaagcttct atcttttcac gatctgcagc atctatcatg
      421 taaacaatag cattgactcc tctgcaatac cgctcccaca tgcttcgaaa tcggggttgt
      481 cctcctatgt cccagatctt tattgtgacg ttacctttag ttaccttcct catgttgaag
      541 cccactgtgg gtatcatatc ttcactgaat tgacctgacg cgatgacatt gacgaaggtg
      601 gtcttgcccg agtactgcag ccccacgagc gtcagctcca tctcttcctt ccagaagagc
      661 gaacggaacc agtccagcag gcgggagatg agcgccagca tggtttatct ccttctaagc
      721 ctgctttttt gtacaaactt gtgatattcc ttcttaaagt taaacaaaat tatttctaga
      781 ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg
      841 agatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
      901 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcggggctcg ccactttcgg
      961 gctcatgagc gcttgtttcg gcgtgggtat
//
