LOCUS       sequence-118139-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  photoactivatable PKA inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Yi JJ, Wang H, Vilela M, Danuser G, Hahn KM
  TITLE     Manipulation of endogenous kinase activity in living cells using 
            photoswitchable inhibitory peptides.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):788-95. doi: 10.1021/sb5001356. 
            Epub 2014 Jun 13.
  PUBMED    24905630
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb5001356";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "788-95"
COMMENT     Sequence Label: mVenus-PA-PKI
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..712
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(41..62)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(302..321)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     649..670
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
       61 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      121 gctgaccctg aagttgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      181 gaccaccctc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
      241 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      301 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      361 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      421 ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
      481 caaggcaaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
      541 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      601 gagctaccag tccaaactga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      661 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact
      721 cagatctcga gaaatgggat ccttggctac tacacttgaa cgtattgaga agaactttgt
      781 cattactgac ccaagattgc cagataatcc cattatattc gcgtccgata gtttcttgca
      841 gttgacagaa tatagccgtg aaaaaatttt gggaaaaaaa ctgcaggttt ctacaaggtc
      901 ctgaaactga tcgcgcgaca gtgagaaaaa ttagagatgc catagataac caaacaga
//
