LOCUS       sequence-118142-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArl14 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARL14-V5-His6
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(56..97)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    117..141
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(736..760)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    801..825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(825..844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(826..844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggctttgtta gcagccggat caaactcaat ggtgatggtg atgatgaccg gtacgcgtag
       61 aatcgagacc gaggagaggg ttagggatag gcttaccttc aagcttcgaa ttgatcacca
      121 ctttgtacaa gaaagctggg tcgttctgct tgaagaacgc caaagtgtct cctcttgatt
      181 tcatgtggct cttcacaaat ccagttaatt tcctgaaccc ctgggccagc ccctcccctg
      241 tgagggcaca gcagggttgc acataccagt tccggtcact gcaaagcttc ttcactttga
      301 acattctggt gatgtcctca gcagtcagag ctccaggcat gtcttgtttg ttggctaata
      361 gaacaacagg cacattttta atgtgttcat tcttcaaaat gtgctcaaac tgtctctgag
      421 actcttccag tcgctgtttg tctgtactgt ccacaacata caccagccca tcggtgttct
      481 cacagtaaca gccccaaaca gttctcattt tttcctgtcc tccaacatcc cagactgtga
      541 gtgaaaaatt cctttccaac tcgatcattt ccacattgaa acctattgta gggatggtgg
      601 taatatcctt agcaagcttt aatttataaa ggagagtaga cttcccagct gagtcaagtc
      661 ccaaaagaag aacttgggct tgtttggttt gcggattttt agaacccagc gaacccatgg
      721 ttctatctcc ttctaagcct gcttttttgt acaaacttgt gatattcctt cttaaagtta
      781 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
      841 attaatttcg cgggaatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc
      901 accggcgcca caggtgcggt tgctggcgcc tatatcgccg a
//
