LOCUS       sequence-118145-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArl11 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARL11-V5-His6
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(45..86)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    106..130
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(737..761)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    802..826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(826..845)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(827..845)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(929..948)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aagccggatc aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg
       61 aggagagggt tagggatagg cttaccttca agcttcgaat tgatcaccac tttgtacaag
      121 aaagctgggt cagatctctt gctgtctccg cgctcagccc catgggctct cgcctgcaga
      181 cacatgcagc tgcgagattt caggaggctc cacaggctct gcagggcctc gggcagcccc
      241 tccccagtga gggcactgca gccccggagc tcccagcggt ggtcctggaa tctctctaga
      301 ctcagcctgt ttctgatctt aagcagcgga agtgcatcag gtgcctcctg cttgttggcc
      361 agcaccaaga aggggacgcc agccatgttg gggtcgttca ggacttctgt gagctcagcc
      421 gccgactcgg gtaagcgggc ttcatctgtg ctgtccagca cgtacacgag gatatctgtg
      481 ccttccagat agtccttcca gctggctctg agcggggcct gccccccaac gtcccagaga
      541 gtcagtgaca cgtgcccagg agctttcaga ggctccacgt tgaaaccaac agtgggcagg
      601 gtctccacca gctggtggcc cttcagcttg taaaggagcg tggtcttgcc cgccgagtcc
      661 aggcccatca tcaccacctg ggcttccgcc ttgtgacctc tggaattcac agaacccatg
      721 gttctatctc cttctaagcc tgcttttttg tacaaacttg tgatattcct tcttaaagtt
      781 aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg
      841 tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc
      901 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      961 cgggctcgcc acttcgggct catgagcgc
//
