LOCUS       sequence-118148-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArfrp with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARFRP1-V5-His6
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    118..142
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(764..788)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    829..853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(853..872)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(854..872)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggctttgtt agcagccgga tcaaactcaa tggtgatggt gatgatgacc ggtacgcgta
       61 gaatcgagac cgaggagagg gttagggata ggcttacctt caagcttcga attgatcacc
      121 actttgtaca agaaagctgg gtccgtgatg tccctctgcc gcggcggccg gtgcacattc
      181 cgcacgacac acttcaccat ccactcgatg ccctcgcgca cccctttgcc tgtgagggcc
      241 gagcaggcct gggtcaggca atcgcgcctg ccgatcttgc tggtgcagtc gctgaaggcc
      301 gtcttgatgt cagggattga gaggcacgtc tccacatcct gcttgttggc cagcaccaag
      361 acggggacac cgcacagcgc ctcgctggtc accaccttct caaacgcctg cttggactca
      421 gccagcctct cctcgtcggt ggagtcaatg acgtagatga cgccgtgaca ctccgcataa
      481 tacttgtccc acaaagactg cagctcttcc tgccctccta agtcccagaa catgagccga
      541 gcctttccca catccacagt gccgatgttt aggcccacgg tggtggtgat tttggataga
      601 ctcatcccct tgtagttctt gttaaatcgg gttttcgact gctccaggaa ggtcgtcttc
      661 ccagcattgt ccaggcccag gatcaggatg cagtactcgt ccttctgaaa catgtacttg
      721 tacaagcccg acagcagcgt gtacatggtt ctatctcctt ctaagcctgc ttttttgtac
      781 aaacttgtga tattccttct taaagttaaa caaaattatt tctagagggg aattgttatc
      841 cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc
      901 tacgccggac gcatcgtggc cggcatcacc g
//
