LOCUS       sequence-118151-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..711
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     regulatory      766..775
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             817..852
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 ccgtcagatc cgctagcgct accggtcgcc accatgagtg cgattaagcc agacatgaag
       61 atcaaactcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt
      121 acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga gggcggacct
      181 ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgcc
      241 aaatatccag acaacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg
      301 gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga cataacaatg
      361 gaaggggaca ctttctataa taaagttcga ttttatggta ccaactttcc cgccaatggt
      421 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt
      481 gatggagtgc tgacgggtga tattcatatg gctttgttgc ttgaaggaaa tgcccattac
      541 cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggctac
      601 cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa caaggttaag
      661 ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg atccggactc
      721 agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcggccac catggctact
      781 caagctgacc tgatggagtt ggacatggcc atggagccgg acagaaaagc tgctgtcagc
      841 cactggcagc agcagtctta cttggattct ggaatccatt ctggtgccac caccacagct
      901 ccttccctg
//
