LOCUS       sequence-118164-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  hArf4 with stop codon in Gateway Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDONR221-ARF4
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(677..776)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(792..809)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(792..808)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gtcctattaa
      121 cgttttgaaa gctcatttga cagccagtca agtccttcat acagaccagt tccttgtgtt
      181 gcacaagtgg cttgaacata ccatgttctg ttacgaagag actgaagccc tagtttatct
      241 gtcatttcac tgatggccat agcatttggc aaatcctgtt tgtttgcaaa aagtagcagc
      301 actgcatctc tcaattcatc taccagaagc attttctgca gctcatctgc tacttcctga
      361 attctttcac gatcgttgct atctaccaca aaaataagac cctgggtatt ctggaagtaa
      421 tgcttccaga gaggcctaat tctatcttga ccaccaacat cccatactgt gaaacaaatg
      481 ttcttatatt ctactgtttc cacattaaaa ccaatggtag gaatggtggt gactatctcc
      541 cctaacttca gtttatacag aatggttgtc ttgccagcag catccaatcc aaccatcaaa
      601 atgcgcatct gcttcttgcc aaatagtcgg gagaagaggg aggagatagt gaggcccatg
      661 gttctatctc cttctaagcc tgcttttttg tacaaagttg gcattataaa aaagcattgc
      721 tcatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttggggc
      781 ccgagcttaa gactggccgt cgttttacaa
//
