LOCUS       sequence-118169-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf6 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARF6
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(635..659)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(691..710)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(692..710)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(755..854)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(784..804)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acaagaattg ggacaactcc agtgaaaagt tcttctcctt tgctagccat tctagatcga
       61 accactttgt acaagaaagc tgggtcctat taagatttgt agttagaggt taaccatgtg
      121 agcccctcat agagtccgtc ccctgaggtg gcacaggagg gctgcacata ccagttcctg
      181 tcccgaatcc gggtcaggcc cagtttctcc tggatctcgt ggggtttcat ggcatcgggc
      241 aggtcctgct tgttggcgaa gatgaggatt atggcgtccc tcatctcccg gtcattgata
      301 atgcggtgca gctcctggcg agcctcatcg atgcggtcgc ggtcggcgca gtccactacg
      361 aagatgagac cttgggtccc agtgtagtaa tgccgccaga gcggccggat cttgtcctgg
      421 ccgcccacat cccatacgtt gaacttgaca tttttgtaag tcaccgtctc cacgttgaaa
      481 cccacagtgg gaatggtggt caccgactgg cccagcttca acttgtacag gattgttgtc
      541 ttgccggccg cgtccaggcc caacatgagg atccgcattt ccttgttccc gaagattttg
      601 gatagcacct tccccatggt tctatctcct tctaagcctg cttttttgta caaacttgtt
      661 tgatcaagct taactagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa
      721 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc
      781 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
      841 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
      901 atccccgtga
//
