LOCUS       sequence-118171-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl2 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL2
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(659..683)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(715..734)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(716..734)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(779..878)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(808..828)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcctatcag tcagctgtga aaatgcggct ggaaatgtca
      121 tccaggagcc agtcgatgcc cggcagcagg ttctccccgg tgacggcgct gcagccctgg
      181 atgcaccagt ggtggctgcg gatggagtcc agctccaggg cctcgcggat ggcgttagag
      241 gacagtgctc caggcaggtc ctgcttatta gcaaagatga ggagggttgc tccggccagg
      301 cgctcctcca ccagcaggct ctggagctcc cgctggcagt cctgcatgcg ctggcggtct
      361 gcgctgtcca ctacccagat gaggccatcg gtgctctcaa agtagttccg ccagtaggac
      421 cgcagggact tctggccacc cacatcccag atgttcagct tgaatcctcg gtgctccagg
      481 gtcttgatgt tgaagcccag cgttggggag atggtgtcga tgtcctcccc attgaacttc
      541 ttcaggatgg ttgtctttcc agcattgtcc aggccaagca tgagcagtcg cagctcccgc
      601 tctttctgct tcatcttctt cagaatggtc aggagcccca tggttctatc tccttctaag
      661 cctgcttttt tgtacaaact tgtttgatca agcttaacta gccagcttgg gtctccctat
      721 agtgagtcgt attaatttcg ataagccagt aagcagtggg ttctctagtt agccagagag
      781 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
      841 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctccca
//
