LOCUS       sequence-118173-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the UBE2S-UBD fusion protein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Bremm A, Freund SM, Komander D
  TITLE     Lys11-linked ubiquitin chains adopt compact conformations and are 
            preferentially hydrolyzed by the deubiquitinase Cezanne.
  JOURNAL   Nat Struct Mol Biol. 2010 Aug;17(8):939-47. doi: 10.1038/nsmb.1873. 
            Epub 2010 Jul 11.
  PUBMED    20622874
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.1873";
            journalName: "Nat Struct Mol Biol"; date: "2010-08"; volume: "17"; 
            issue: "8"; pages: "939-47"
COMMENT     Sequence Label: pGEX6P1-UBE2S-UBD
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    106..117
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 cagatcgtca gtcagtcacg atgcggccgc ttatgtcttc tgcatcttca gcatgtcgat
       61 gccgaagtgg gacaggtgct cagccaggct ggggtccagg accatgtcat cctcatcata
      121 tgagtacacg tcagctccat caggggtgat ggtgcccagc ttgacagcta acgggtagcc
      181 tgtctctcgg tagtgctcca cagcgtggtt gttgccccca ctgccatcga agtagcgtcg
      241 cccacagagg atggagccat cagtcaggtt gagccacagg ttctctctca tgtcacactt
      301 ggagcacttc cagccacagg gagggattcg agcagggttg tccaactgct tgaggctgaa
      361 ggcatgctta gacacctgcc gtaccatggg accctcagcc cctcccgggc cccctggggc
      421 cccagggtcg gtggaggaag cttcagtgcc actggccagg gcccgaccgg cttcggccct
      481 gccgctgggc ccgccggcgc ccccgtggat ctctgtgagc agacgggccc gagctgcata
      541 ctcctcgtag ttctccaaga gcaggcggcc cgcctcctcg ttgagtgcag actcggggtt
      601 agggtggatc agcaggcact tgatggtcag cagtacgtgt cggatgccca gctcagccgt
      661 ccagtccctc ttgagcacgt tgacgcagat ctcgccattg gcgcccacgt tcgggtggaa
      721 gatcttggtc aggaagtag
//
