LOCUS       sequence-118174-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl3 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL3
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(656..680)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(712..731)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(713..731)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(776..977)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(805..825)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acaagaattg ggacaactcc agtgaaaagt tcttctcctt tgctagccat tctagatcga
       61 accactttgt acaagaaagc tgggtcctat tatttcttct ttgcattgac atttttgcag
      121 acccagttca tgccatcctg aacgccctct cctgtgagag ctgagcaaga ctggatctgc
      181 cagactcggt cgcggatggt atgcaggttc agtccttctg caatttcaga ggcaggggct
      241 gctgtgagca aatcctgctt attagcaaag atgagcactg gcacacaact tagtttttct
      301 tcctccagta attccgctag ttcctgaccc gtctcttcaa atctttttct gtctgcactg
      361 tcgattacat atataagaat atcggtattt tcaaaataat tcttccagta tggtctgatt
      421 ttcctctgtc caccaatgtc ccatacattc agtttaaaac cttgtgattg tacacttttg
      481 atgttgaaac cctgtgtagg tgtgatgtgg ctgatgtctt cagatgcaag ctgcttcaga
      541 agagtggtct tgccagcatt atccaagccc aggagaagta ttctcacctc ctggtctggt
      601 gcacttttca acttgcgcaa aattgagagc aagcccatgg ttctatctcc ttctaagcct
      661 gcttttttgt acaaacttgt ttgatcaagc ttaactagcc agcttgggtc tccctatagt
      721 gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc
      781 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
      841 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
      901 caatggggtg gagacttgga aatcccgtga gtcaaaccgc tatccacgcc cattgatgta
      961 ctgccaaacc gcatcaccat ggtaatagcg atgacta
//
