LOCUS       sequence-118176-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl4 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL4
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(707..731)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(763..782)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(764..782)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(827..926)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(856..876)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcctatcat cttttctttt tctgttgccg caacattttt
      121 cttcttttaa tgatcatatc atgtagtttc tcaagtcctt cctttaggcc atctcctatg
      181 attgcacagg taggctgcaa atgccaagga gttgatgagc tcagttcacc cattgctaac
      241 aatttctcaa tttctgaaag tgacaatgag ttcctcaaat cttgtttgtt agcaactata
      301 agtacaggga ctccctgatt ttctgatatc ctagttattt tgtgaagttc agttttggct
      361 tcttccatcc tttcgacatc aacagagtcc acaacaaata caatgccatc tgtgcatctg
      421 gtatatgact tccacagtgg ccttaatttc tcctgaccac ctacatccca gaagtgaaaa
      481 gtgactgttt tagaatttcc caaggttacc ttaattttct cagtgttaaa tcctttggta
      541 ggtacggtat ttacaaattc attgaactgc agcctgtata agacagttgt ctttccagca
      601 cagtccaaac ccagaataac aatgtggaaa gactgaaatg aaggcaggtt ggacaggata
      661 gaagtctggt ctgacagccc attccccatg gttctatctc cttctaagcc tgcttttttg
      721 tacaaacttg tttgatcaag cttaactagc cagcttgggt ctccctatag tgagtcgtat
      781 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
      841 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      901 attttggaaa gtcccgttga ttttggtgcc aaacaaactc ccattg
//
