LOCUS       sequence-118177-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NleL (170-782) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Hospenthal MK, Freund SM, Komander D
  TITLE     Assembly, analysis and architecture of atypical ubiquitin chains.
  JOURNAL   Nat Struct Mol Biol. 2013 May;20(5):555-65. doi: 10.1038/nsmb.2547. 
            Epub 2013 Apr 7.
  PUBMED    23563141
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2547";
            journalName: "Nat Struct Mol Biol"; date: "2013-05"; volume: "20"; 
            issue: "5"; pages: "555-65"
COMMENT     Sequence Label: pMCSG17-NleL
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..63
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gaaggagata tacatatgaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
       61 agcccatctt ccggcgtaga tctgatgtcc cctatactag gttattggaa aattaagggc
      121 cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg
      181 tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt
      241 cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata
      301 cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt
      361 tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt
      421 aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg
      481 ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac
      541 ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg
      601 ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac
      661 ttgaaatcca gcaagtatat agcat
//
